Dextro-ciprofibrate

ABSTRACT

Dextro-ciprofibrate or a pharmaceutically acceptable salt thereof is disclosed.

BACKGROUND OF THE INVENTION Field of the Invention

The invention relates to dextro-ciprofibrate, which is useful as a serumlipid lowering agent, and method of use and compositions thereof.

SUMMARY OF THE INVENTION

In a first composition of matter aspect the invention isdextrociprofibrate, which is(d)-(+)-2[4-(2,2-dichlorocyclopropyl)phenoxy]-2-methylpropanoic acid, ora pharmaceutically acceptable salt thereof.

In a process aspect the invention is the method of reducing theconcentration of serum lipids in a mammal comprising administering tothe mammal an effective serum lipid lowering amount ofdextro-ciprofibrate or a pharmaceutically acceptable salt thereof.

In a second composition of matter aspect the invention is a compositionwhich comprises an effective serum lipid lowering concentration ofdextrociprofibrate or a pharmaceutically acceptable salt thereof and apharmaceutically acceptable vehicle.

DETAILED DESCRIPTION OF THE INVENTION INCLUSIVE OF THE PREFERREDEMBODIMENTS Preparation of Dextro-ciprofibrate

In a first method of preparation dextro-ciprofibrate is prepared byresolving racemic ciprofibrate by fractional crystallization of a saltthereof with an optically active amine. The preferred optically activeamine is (R)-(+)-α-methylbenzylamine. The preferred solvent isacetonitrile.

In a second method of preparation dextro-ciprofibrate is prepared byfirst resolving 4-(2,2-dichlorocyclopropyl)aniline using (-)-tartaricacid and then synthesizing dextro-ciprofibrate from the resulting(+)-4-(2,2-dichlorocylopropyl)aniline by the steps ofdiazotization-hydrolysis using sodium nitrite in aqueous sulfuric acidat 0°-10° C., then condensing the resulting(+)-4-(2,2-dichlorocyclopropyl)phenol with acetone and chloroform inaqueous alkali at 0°-100° C.

The following examples illustrate the method of preparation.

EXAMPLE 1(1)-(-)-2-(4-(2,2-Dichlorocyclopropyl)phenoxy)-2-methylpropionic acid

A solution of (R)-(+)-α-methylbenzylamine (28.5 ml) in acetonitrile (50ml) was added to a solution of2-(4-(2,2-dichlorocyclopropyl)phenoxy)-2-methylpropionic acid (1)(128.15 g) in acetonitrile (1.45 L) at room temperature and leftstanding for 3 hours. The resulting crystals of the salt of(R)-(+)-α-methylbenzylamine with (1), A (23.9 g), were removed byfiltration. The filtrate was concentrated by evaporation under reducedpressure and a second crop of the salt, B (12.0 g), removed byfiltration. A third crop, C (28.6 g), was obtained similarly and theresidual filtrate was evaporated to dryness under reduced pressure toyield a gum D.

The crystalline material C was suspended in dilute hydrochloric acid(200 ml, 2M) and extracted three times with diethyl ether. The combinedorganic phases were washed consecutively with distilled water and brinebefore being dried over anhydrous magnesium sulphate. The organic phasewas then removed under reduced pressure to afford a white solid (22.89g) with [α]_(D) ²¹ (EtOH) of -7.92°.

The samples A, B and D were extracted similarly to afford white solidswith mass and [α]_(D) ²¹ (EtOH) respectively; (15.81 g [α]_(D) ²¹+32.07°, (8.34 g) [α]_(D) ²¹ +25.16° and (70.50 g) [α]_(D) ²¹ -9.03°.

The white solids resulting from the above hydrolyses of salt samples Cand D were combined and dissolved in acetonitrile (975 ml). To thissolution was added a solution of (S)-(-)-α-methylbenzylamine (25 ml) inacetonitrile (75 ml) at room temperature. After 2 hours the crystallinesalt of partially resolved (1) with (S)-(-)-α-methylbenzylamine (42.21g) was removed by filtration. This material was suspended in dilutehydrochloric acid (350 ml, 2M) and extracted three times with diethylether. Combined organic phases were washed sequentially with water andbrine before being dried over anhydrous magnesium sulfphate. The organicphase was removed by evaporation under reduced pressure to afford awhite solid (27.69 g) with [α]_(D) ²¹ (EtOH) of -31.02°.

The above salt formation between partially resolved (1) and(S)-(-)-α-methylbenzylamine and subsequent hydrolysis was repeated afurther seven times until material (514 mg) with [α]_(D) ²¹ of -67.11°was obtained. This material (400 mg) was combined with a similarlyderived sample (440 mg) of [α]_(D) ²¹ of -67.83° and dissolved indiethyl ether (50 ml). This solution was dried over anhydrous magnesiumsulphate and the organic phase removed by evaporation under reducedpressure to afford, as an amorphous white solid,(1)-(-)-2-(4-(2,2-dichlorocyclopropyl)phenoxy)-2-methylpropionic acid(737 mg), m.pt. 71.6°-75.2° C., (Found: C, 53.94; H, 4.91%: C₁₃ H₁₄ Cl₂O₃ requires C, 54.00: H, 4.88%).

(d)-(+)-2-(4-(2,2-Dichlorocyclopropyl)phenoxy)-2-methylpropionic acid

The white solids resulting from the above hydrolyses of salt samples Aand B were combined and salt formation between partially resolved (1)and (R)-(+)-α-methylbenzylamine and subsequent hydrolysis was repeated afurther eight times until material (452 mg) with [α]_(D) ²¹ of +64.31°was obtained. This was combined with a similarly derived sample (890 mg)with [α]_(D) ²¹ of +62.71°. This combined sample was further crystalisedas the salt with (R)-(+)-α-methylbenzylamine and subsequently hydrolysedto afford, as an amorphous white solid,(d)-(+)-2-(4-(2,2-dichlorocyclopropyl)phenoxy)-2-methylpropionic acid(630 mg), m.pt 71.6°-75.2° C., (Found: C, 54.05; 4.90%: C₁₃ H₁₄ Cl₂ O₃requires C, 54.00: H, 4.88%) with [α]_(D) ²¹ (EtOH) of +66.70°.

The enantiomeric purity of the above samples was measured by analyticalhigh performance liquid chromatography (hplc) of the correspondingmethyl esters on a Chiracel OA column eluted with methanol/water(21:19). The methyl esters were prepared as exemplified below.

(1)-(-)-2-(4-(2,2-Dichlorocyclopropyl)phenoxy)-2-methylpropionic acidmethyl ester

(1)-(-)-2-(4-(2,2-Dichlorocyclopropyl)phenoxy)-2-methylpropionic acid(30 mg) in methanol (4.5 ml) and concentrated sulphuric acid (2 drops)were heated under reflux for 1.5 hours. The solution was concentrated byevaporation under reduced pressure and the residue dissolved in diethylether (10 ml), washed twice with saturated aqueous sodium hydrogencarbonate, water and brine, before being dried over anhydrous magnesiumsulphate. The organic phase was removed by evaporation under reducedpressure to afford the desired(1)-(-)-2-(4-(2,2-dichlorocyclopropyl)phenoxy)-2-methylpropionic acidmethyl ester as a white solid (22 mg).

Under the hplc conditions described above, the(1)-(-)-2-(4-(2,2-dichlorocyclopropyl)phenoxy)-2-methylpropionic acidmethyl ester was analysed as having an excess of the desired enantiomerover the racemate of 95.5%. The analogously prepared(d)-(+)-2-(4-(2,2-dichlorocyclopropyl)phenoxy)-2-methylpropionic acidmethyl ester was analysed by the same method as having an excess of thedesired enantiomer over the racemate of 94.0%.

EXAMPLE 2 Synthesis of(-)-2-[4-(2,2-Dichlorocyclopropyl)phenoxy]-2-methylpropanoic acid4-(2,2-Dichlorocyclopropyl)aniline

A solution of (+)-tartaric acid (33.3 g, 0.22 mol) in methanol (1 L),was added to a solution of 4-(2,2-dichlorocyclopropyl)aniline (90 g,0.44 mol) in methanol (1 L) and warmed to 50° C. The solution wasallowed to cool to room temperature overnight. The resulting crystalswere removed by filtration and added to an aqueous solution of sodiumhydroxide (1M, 1 L) before being extracted with diethyl ether (3×200ml). The combined organic phases were washed with distilled water beforebeing dried over anhydrous magnesium sulphate. The solvent was thenremoved by evaporation under reduced pressure to afford partiallyresolved (-)-4-(2,2-dichlorocyclopropyl)aniline (23.7 g) with an [α]_(D)²¹ (EtOH) of -96°.

The above was repeated with further batches of4-(2,2-dichlorocyclopropyl)aniline until a quantity (142.4 g, 0.70 mol)of partially resolved material was accumulated. This material wasdissolved in methanol (1.57 L) and added to a solution of (+)-tartaricacid (97.7 g, 0.65 mol) in methanol (1.57 L). The solution was allowedto stand overnight at room temperature. The resulting crystals wereremoved by filtration and added to an aqueous solution of sodiumhydroxide (1M, 1.5 L) before being extracted with diethyl ether (4×200ml). The combined ethereal extracts were washed with water, and thenbrine, before being dried over anhydrous magnesium sulphate. The organicphase was removed by evaporation under reduced pressure to affordresolved (-)-4-(2,2-dichlorocyclopropyl)aniline with an [α]_(D) ²¹(EtOH) of -104°.

A sample of this material was analysed by high performance liquidchromatography on a ChiralCel column (CA-1) in ethanol:water (7:3) asmobile phase, and determined to have an enantiomeric ratio of 95:5 infavour of the (-)-enantiomer.

(-)-4-(2,2-Dichlorocyclopropyl)phenol

(-)-4-(2,2-Dichlorocyclopropyl)aniline (5 g, 0.024 mol) from the aboveresolution was dissolved in concentrated sulphuric acid (25 ml) andadded in aliquots (1 ml) to iced water (100 ml). The mixture was allowedto stand overnight and the resulting precipitate then removed byfiltration. The solid was suspended with stirring in sulphuric acid(0.05M, 70 ml) whilst being cooled to below 5° C. and a solution ofsodium nitrite (2.2 g, 0.032 mol) in sulphuric acid (0.05M, 30 ml)added. After stirring for 1.5 hours the remaining precipitate wasdissolved by addition of concentrated sulphuric acid (2.4 ml) andstirring maintained for a further 1.5 hours. The pH of the solution wasthen adjusted to 2 by addition of an aqueous solution of sodiumhydroxide (10M) before being added to a stirred mixture of cupricnitrate hemipentahydrate (50 g, 0.21 mol) and sodium ascorbate (2.3 g,0.012 mol) in distilled water (440 ml) and diethyl ether (150 ml). Themixture was stirred for 10 minutes before the organic phase was removedand the aqueous phase extracted with diethyl ether (3×100 ml). Thecombined organic phases were washed with distilled water, dried overanhydrous magnesium sulphate and the solvent removed by evaporationunder reduced pressure to afford the crude(-)-4-(2,2-dichlorocyclopropyl)phenol (4.6 g) which was used directly inthe following reaction.

(-)-2-[4-(2,2-Dichlorocyclopropyl)phenoxy]-2-methylpropanoic acid

The crude (-)-4-(2,2-dichlorocyclopropyl)phenol (4.6 g) from theprevious reaction was dissolved in acetone (63.2 ml) and added slowly toa solution of sodium hydroxide (6.7 g, 0.17 mol) in acetone (42 ml)under reflux. After 15 minutes, chloroform (1.5 g, 0.013 mol) in acetone(25 ml) was added at a rate which maintained a gentle reflux. After onehour, the solvent was removed by evaporation under reduced pressure andthe resulting solid partitioned between distilled water (350 ml) anddiethyl ether (100 ml). The organic phase was removed and the aqueousphase acidified with dilute hydrochloric acid and extracted with diethylether (2×100 ml). The combined ethereal extracts were washed with water,dried with anhydrous magnesium sulphate and the solvent removed byevaporation under reduced pressure to afford the crude product (3.5 g).This material was chromatographed on silica gel eluting with diethylether/hexane/glacial acetic acid (4:15:1) to afford(-)-2-[4-(2,2-dichlorocyclopropyl)phenoxy]-2-methylpropanoic acid (0.5g) with properties identical with that obtained by the describedresolution of racemic material with (-)-α-methylbenzylamine.

A sample of this material was methylated and analysed by chiral HPLC, asdescribed for the resolved material, and found to have an enantiomericratio of greater than 95:5 in favour of the (-)-enantiomer.

Synthesis of(+)-2-[4-(2,2-Dichlorocyclopropyl)phenoxy]-2-methylpropanoic acid.(+)-4-(2,2-Dichlorocyclopropyl)aniline

4-(2,2-Dichlorocyclopropyl)aniline (50 g, 0.25 mol), enriched with the(+)-enantiomer following partial resolution with (+)-tartaric acid asdescribed above, was dissolved in methanol (500 ml) to which was added asolution of (-)-tartaric acid (22.7 g, 0.15 mol) in methanol (500 ml).The solution was allowed to stand overnight and the resulting crystalsremoved by filtration. These crystals were added to an aqueous solutionof sodium hydroxide (1M, 350 ml) and the solution extracted with diethylether (3×100 ml). The combined ethereal extracts were dried overanhydrous magnesium sulphate and the solvent removed by evaporationunder reduced pressure. The residue (18.1 g) was treated analogously afurther two times until (+)-4-(2,2-dichlorocyclopropyl)aniline (7.8 g)was obtained with an [α]_(D) ²¹ (EtOH) of +107° C.

(+)-4-(2,2-Dichlorocyclopropyl)phenol

(+)-4-(2,2-Dichlorocyclopropyl)aniline (3 g, 0.015 mol) from theprevious resolution was dissolved in concentrated sulphuric acid (15 ml)to which was added iced water (30 ml). With the reaction temperaturemaintained below 5° C. a solution of sodium nitrite (1.3 g, 0.019 mol)in distilled water (15 ml) was added. After a further 5 minutes, urea(0.27 g, 0.0045 mol) was added to eliminate excess nitrous acid. The pHof the mixture was then adjusted to 3 by addition of an aqueous solutionof sodium hydroxide (10M) whilst the temperature of less than 5° C. wasmaintained. This mixture was then added slowly to a vigorously stirredmixture of cupric nitrate hemipentahydrate (60 g, 0.26 mol) and sodiumascorbate (2.8 g, 0.014 mol) in distilled water (530 ml) and diethylether (200 ml). After 5 minutes the organic phase was removed and theaqueous phase extracted with diethyl ether (3× 100 ml). The combinedorganic phases were washed with distilled water, dried over anhydrousmagnesium sulphate and the solvent removed by evaporation under reducedpressure to afford crude (+)-4-(2,2-dichlorocyclopropyl)phenol (3.1 g)which was used directly in the following reaction.

(+)-2-[4-(2,2-Dichlorocyclopropyl)phenoxy]-2-methylpropanoic acid

The crude (+)-4-(2,2-dichlorocyclopropyl)phenol (3.1 g) from theprevious reaction was dissolved in acetone (42 ml) and added slowly to asolution of sodium hydroxide (4.5 g, 0.11 mol) in acetone (27 ml) undergentle reflux. After 5 minutes, a solution of chloroform (1.0 g, 0.008mol) in acetone (17 ml) was added at a rate so as to maintain thisreflux. After one hour the solvent was removed by evaporation underreduced pressure and the resulting solid partitioned between distilledwater (150 ml) and diethyl ether (3×50 ml). The aqueous phase wasacidified with dilute hydrochloric acid and extracted with diethyl ether(3×50 ml). The combined organic phases were washed with distilled water,dried over anhydrous magnesium sulphate and the solvent removed byevaporation under reduced pressure. The resulting solid waschromatographed on silica gel eluting with diethyl ether/hexane/glacialacetic acid (4:15:1) and the product recrystallised from hexane toafford (+)-2-[4-(2,2-dichlorocyclopropyl)phenoxy]-2-methylpropanoic acid(460 mg) with properties identical to that obtained by resolution ofracemic material with (+ )-α-methylbenzylamine as described above.

A sample of this material was methylated and analysed by chiral HPLC, asdescribed for the resolved material, and found to have an enantiomericratio of greater than 95:5 in favour of the (+)-enantiomer.

Biological Properties of Dextro-ciprofibrate

Racemic ciprofibrate, which is described by U.S. Pat. No. 3,948,973 andwhose disclosure is incorporated herein by reference and is an effectiveserum lipid lowering agent in humans, has a half-life by about 100 hr.in the rat. Dextro-ciprofibrate has a half-life of 160 hr. in the rat.This difference will result in more prolonged serum lipid loweringeffect, thus permitting less frequent dosing and further resulting inbetter patent compliance by dextro-ciprofibrate than by racemicciprofibrate. The daily dosage is from about 50 mg. to about 500 mg.

The Compositions

The compositions in accordance with the second composition of matteraspect of the invention can be prepared for oral, parenteral, rectal orvaginal administration and can be in solid or liquid dosage formincluding capsules, tablets, suppositories, solutions, suspensions andemulsions. Conventional pharmaceutically acceptable vehicles andtechniques are used in preparing these dosage forms.

We claim: 1.(d)-(+)-2-[4-(2,2-Dichlorocyclopropyl)phenoxy)-2-methylpropionic acid ora pharmaceutically acceptable salt thereof.
 2. The method of reducingthe concentration of serum lipids in a mammal comprising administeringto the mammal an effective serum lipid lowering amount ofdextro-ciprofibrate or a pharmaceutically acceptable salt thereof.
 3. Acomposition which comprises an effective serum lipid loweringconcentration of dextro-ciprofibrate or a pharmaceutically acceptablesalt thereof and a pharmaceutically acceptable vehicle.
 4. The processof preparing a compound according to claim 1 comprising resolvingracemic ciprofibrate by fractional crystallization of a salt thereofwith an optically active amine.
 5. The process of preparing a compoundaccording to claim 1 comprising first resolving4-(2,2-dichlorocyclopropyl)aniline using (-)-tartaric acid and thensubjecting the resulting (+)-4-(2,3-dichlorocylcopropyl)aniline todiazotization-hydrolysis using sodium nitrite in aqueous sulfuric acidat 0°-10° C. and then condensing the resulting(+)-4-(2,2-dichlorocyclopropyl)phenol with acetone and chloroform inaqueous alkali at 0°-100° C.